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Something went wrong. Please try again later. Each sequenced fish is homozygous, and was crossed to make genetically identical and heterozygous F1 hybrids: with one intact AB and TU chromosome from each parent.
These F1s were crossed to make F2s, which should have one crossover per chromosome. Clark et al. Due to the nature of this cross, we believe that the SAT line should be largely free of embryonic lethal mutations, because these should have been selected against by the doubled haploid process any remaining lethal mutations might have been complemented by wild-type maternal mRNA or protein. Similarly trans-heterozygous lethal alleles should not exist in the F1s.
For F2s and subsequent generations, the genome should be reconstructable by dense genotyping, e. Thus, this strain is genetically defined, and the individual genomes are definable. Furthermore, it is largely free of lethal and deleterious alleles, and should possess hybrid vigor enabling use as a wild-type line and maintenance as a strain. We particularly recommend this line for use where the knowledge of exact genome sequence is important, e.